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Filamentous Identification New training program
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Identification: Relatively large, non-motile filaments (100-500 µm). Straight, smoothly
curved or bent filaments with no branching. Cells are square to rectangular
(1.2-1.6 x 1.5-2.5 µm) and contained in a tight fitting sheath. There are no
indentations at the cell septa. Filaments are found within the floc structure
causing open, lacy floc or radiating outwards causing inter-floc bridging. The
filament staining is Gram positive or Gram variable when in the floc structure
and Neisser negative with Neisser positive granules observed infrequently.
Neisser positive slime coat frequently observed in industrial wastes. Usually
easy to identify due to its flat, ribbon like structure with twists that make it
difficult to focus on more than a few cells at a time under phase contrast.
Intracellular granules rarely observed. No sulfur granules. Can be heavy
attached growth when observed inside the floc structure. No attached growth when
extending into the bulk solution (usually when in a rapid growth phase, watch
out for this, it usually means significant trouble). Sheath is difficult to
detect except when missing cells.
Similar Organisms: Type 0675 is similar although smaller in diameter and is usually more Gram positive. Type 021N is similar when in the bulk solution and with no attached growth, although Type 021N has no sheath. Environment: This filament is usually found in environments where there low F/M with a long MCRT. It can also be found in low nutrient environments. Very rarely is it a problem filament. Usually it causes a nice backbone for the floc structure. Control: F/M can be changed by increased sludge wasting, changing from complete mix to
plug flow or use of a
Rank: 0041 ranks 4th in number of predominance. Common in over 50% of sludge in low abundance. Can be beneficial at low abundance as a backbone structure for the floc.
Why does it have all that attached growth? Usually only filaments with a sheath get attached growth. The attached growth usually means it has been around a long time in the system. For more information on Filamentous Identification More photos to come. . . If you need more information on our Filamentous ID Training CD or on Internet training on Filamentous bacteria, causes and controls How and why on Wastewater Biomass Analyses
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For more information on Filamentous Identification More photos to come. . . Copyright ©2003 Environmental Leverage Inc. All rights reserved. |
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