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Finally new Release

Filamentous Identification

"the Easy Way"

New training program

 

Nostocoida limicola II

Identification:

Medium length , non-motile filaments (100-200 µm). Bent and irregularly coiled filaments with incidental true branching. Knots sometimes seen. Cell septa are clear with indentations. Cells are oval or disc shaped (1.2-1.4 µm). Filaments are found within the floc structure but may occur in the bulk solution. The filament staining is variable, it is usually Gram negative but sometimes positive and Neisser positive. Usually easy to identify due to its Neisser staining properties. Stains entirely purple and looks like stacked discs (or hockey pucks). In industrial wastes, an organism that is Gram negative and Neisser negative occurs. There is no sheath and there are no sulfur granules. Poly-ß-hydroxybutric acid (PHB) granules are frequently observed as dark intracellular granules. Attached growth is usually uncommon.

Three subtypes are known. Resembles M. parvicella except in its Neisser staining properties.

Environment:

This filament is usually found in environments where there is low DO or low F/M and the presence of organic wastes. Wastes containing starch seem more selective to this filament. Bulking is more common in industrial wastes. The filament appears to be facultative fermentative, which is unique for most filaments.

Control:

Manipulation of F/M (usually an increase) and DO concentration can be used to control the filaments. A selector may be used and chlorination. System changes include changing from a complete mix to plug flow aeration basin configuration.

Rank:

N. limicola ranks 12th in number of predominance in industry. Typically not found in kraft mills. Common in municipalities.

 

 

  

Knots are often common and visible

200x Bright field

Bright Field 400x

look at the knot formed above

Neisser Positive with easy to see segmentation

Gram Negative 1000x

 

The Neisser staining always makes it easy to identify N. limicola

Many knots in this photo

Here it looks like a bit of branching is starting

rare, but possible

The twists, kinks and knots usually make it easier to identify than the similar type 021N.

Both have no sheath and irregular cells, but Type 021N has a more flat, ribbon like twist, while N. limicola has more twists and turns and knots.

The real dead give away though, if all else fails, is the Neisser stain. Type 021N is negative and N. limicola is positive.

For more information on Filamentous Identification

You can see on this photo, two separate strains of N. limicola. One stains only purple at the segments, and the other stained darker and most of the whole filament. Both had clearly visible indentations and segments.

 

 

 

 

 

 

More photos to come. . .

For more information on Filamentous Identification

N. Limicola I

N. Limicola III

 

More photos to come. . .

If you need more information on our Filamentous ID Training CD

or on Internet training on Filamentous bacteria, causes and controls

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