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Mystery Bug of the Month

August-07

Tetrads. . . . . Indications that you have nutrient Deficiency, probably nitrogen

OK, these are often overlooked and many times you need to be at 400x or 10000x. If you have these in your plant, you probably have serious TSS problems in your effluent. If you see this in your slide under the microscope, it means you need to examine your plant and check for nutrient deficiency, probably nitrogen. Check your influent loading vs. N and P levels. If you are a food plant, check swings on the day shift vs. the night shift. You may have a high TSS or get false BOD readings in your final effluent when these are present.

These photomicrographs were taken from a wastewater sample. Most of the objectives used were 100 and 400x.

Come take a closer look at the amazing world under the microscope!





40x Even at low power, you can see how these can impact TSS levels	100x
When you see this with high TSS, but a lack of zooglea, high polysaccharide coating it almost always is just nitrogen deficiency

Low power, yet the distinct large cells and clusters make them easy to pick out even without staining once you know what to look for
When you see this, but high polysaccharide coating, zooglea and many irregular cell clusters along with the tetrads, you probably just got hit with a very high BOD loading and are N as well as P deficient in your system
	You can see there are quite a few different species of bacterial clusters that are present when there is nutrient deficiency in a system
The tetrads are the largest, all indicate a problem with nutrients, the tetrads usually nitrogen
1000x Under Neisser stains, they are bright purple and easy to pick out	1000x Here you can even see the excess slime in the sample







A papermill with high TSS levels due to nutrient deficiency




	More information and photos on Tetrads and Cyanobacteria



Typically found in a system with a sudden high BOD loading, with nutrient deficiency Type 021N and zooglea may also be found in the system when these are present	Bacteria; Proteobacteria; Cyanobacteria

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More to come soon!

Enlargements: If you liked these pictures and want to see additional microorganisms check out the pages below.

Amoeba

Flagellates

Free Swimming Ciliates

Eating under the microscope- Dinner time!

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