Well, now it looks like we have lot of jelly like material mixed in with the floc and bacteria

1000x gram stain

400x Bright field fingered zooglea

1000x Bright Field amorphous zooglea

1000x Gram stain

1000x Gram stain

1000x Neisser stain notice the cells stain purple, but the slime stains brown

1000x  Bright field

1000x Neisser stain

Phase Contrast

400x Bright Field

Hard to believe this is nature!

Lacto-Phenol Cotton Blue stain makes it easy to pick out details on a wet mount

Phase contrast notice how the zooglea on the left is a lot lighter in color than the brown floc structures

1000x Gram stain

1000x Gram stain

Here you can see the fingers stretching out

1000x Gram stain

1000x Gram stain very high levels of polysaccharide coating. Imagine trying to dewater that

1000x Gram stain

Phase contrast

Phase contrast

1000x Neisser stain fingered zooglea

1000x Neisser stain colonies

Phase contrast

1000x Gram stain

1000x Gram stain

1000x you can  see how the slime keeps the cells apart. . . . .

200x Bright Field

1000x Phase contrast

1000x Bright Field

1000x Bright Field

Wow, just changing the size makes everything look completely different!

 

Always zoom in under the microscope to see more details and a totally different perspective.

 

You might be surprised what you find! It also makes it easier to identify when you come in closer and can see more details.